The BioSemi ActiveTwo system is a state-of-the-art, modular electrophysiological recording platform developed specifically for high-resolution and high-density acquisition of biopotential signals in human research. With a legacy rooted in innovation, the ActiveTwo system builds upon the trusted design principles of its predecessor, the ActiveOne, offering substantial improvements in signal fidelity, configurability, scalability, and user flexibility.
Engineered for uncompromised scientific precision, ActiveTwo has become the gold standard for researchers around the globe conducting studies in cognitive neuroscience, neurophysiology, psychophysiology, brain-computer interface (BCI), sleep science, auditory research, and multimodal brain-body interaction.
At the core of the system is the ActiveTwo AD-Box, which houses dedicated 24-bit Delta-Sigma analog-to-digital converters (ADCs) for each electrode channel, ensuring optimal linearity, a high dynamic range, and the ability to record the full frequency spectrum of physiological signals without distortion. Unlike conventional amplifier systems that often rely on analog filtering and shared ADCs, the fully digital architecture of ActiveTwo eliminates inter-channel crosstalk and minimizes noise through galvanic isolation and fiber-optic data transmission.
The system’s full DC coupling, high input impedance, and wide input range (±262 mV per channel) make it particularly well-suited for capturing slow cortical potentials, DC shifts, and high-amplitude signals with excellent fidelity—capabilities that are essential for modern EEG and ERP research.
Thanks to its modular and upgradeable design, researchers can configure ActiveTwo to match their experimental setup, with options ranging from 8 to 256 channels, plus additional slots for auxiliary sensors. The system can grow as your research evolves, supporting increasingly complex paradigms without needing to replace hardware.
The ActiveTwo system is suitable for a wide range of research applications, including:
The BioSemi ActiveTwo system is uniquely positioned as the only commercial EEG/ECG acquisition platform capable of supporting true high-resolution Body Surface Potential Mapping (BSPM)—a technique that requires dense, multi-site, high-fidelity recordings across the entire torso or scalp. While traditional ECG systems are limited to 12 leads and conventional EEG setups often struggle with high channel counts and spatial uniformity, the ActiveTwo’s modular architecture and ultra-clean signal pathway allow researchers to overcome these limitations and capture full-body electrical activity with exceptional precision.
With support for up to 256 active electrodes, each digitized individually at 24-bit resolution with full DC coupling, the ActiveTwo offers the temporal and spatial resolution necessary for accurate mapping of electrical activity over the heart, brain, or muscle surfaces. The system’s wide dynamic range, low noise floor, and absence of analog filters make it especially suited for the subtle signal variations required in cardiac and neurological mapping.
Unlike conventional amplifiers, BioSemi’s active electrode technology ensures minimal signal degradation even with long leads and high electrode counts, reducing common-mode artifacts and improving signal integrity. Electrodes can be placed freely over the thorax, back, or head, enabling customized electrode montages for cardiac BSPM, epicardial simulations, or detailed scalp topographies.
In BSPM applications—such as inverse ECG modeling, cardiac isopotential mapping, or advanced neuromodulation studies—signal timing, spatial density, and amplitude linearity are critical. The ActiveTwo delivers on all fronts, offering per-electrode digitization, fiber-optic data isolation, and robust compatibility with inverse modeling software through open .BDF data formats.
The ActiveTwo system is available in multiple configurations to support a wide range of research domains, from high-density EEG to auditory evoked potentials and body surface mapping. Each configuration is built on the same modular amplifier platform and can be tailored with appropriate accessories and software integrations. It is a highly scalable configuration that supports research-grade EEG acquisition with very high signal fidelity and minimal noise. Ideal for standard EEG research with the flexibility to extend into multimodal designs.
The standard ActiveTwo configuration for high-resolution EEG acquisition. It includes up to 256 EEG channels, 8 auxiliary EXG channels for signals such as ECG, EMG, or EOG, and 7 auxiliary channels (for external microphones and other sensors).
Designed specifically for auditory neuroscience and fast evoked potential research, the hypersampling version supports sample rates up to 262,144 Hz on selected channels, making it suitable for ABR, ASSR, and other high-temporal-resolution paradigms. Unmatched temporal resolution in a human EEG system. Enables precise alignment of electrophysiological signals with auditory stimuli, making it uniquely suitable for ABR and other time-critical paradigms.
This configuration is optimized for non-invasive cardiac mapping using a high number of electrodes distributed across the torso. Supports up to 195 channels when configured with multiple amplifiers and is tailored for integration with torso geometry models. One of the only modular EEG amplifier platforms that can be adapted for BSPM. Supports very high channel densities and spatial mapping precision critical for cardiac signal localization.
This setup is tailored for surface electromyography (sEMG) research, offering high input bandwidth and dynamic range suitable for capturing muscle activity at fine temporal and spatial scales. Combines low-noise EMG signal acquisition with flexibility in sensor placement and referencing. Particularly effective in research settings requiring co-registration of EMG with EEG or other modalities.
Important Note: This product is for research applications only. Not a medical device as defined in Regulation (EU) 2017/745 (EU MDR), Swiss Medical Devices Ordinance (MedDO, SR 812.213), or FDA 21 CFR Part 860. Not designed or intended to be used for diagnosis, treatment of disease or any other medical purposes.
